SCFAtPP2-B11 modulates ABA signaling by facilitating SnRK2.3 degradation in Arabidopsis thaliana

• DOI: 10.1371/journal.pgen.1006947
• Publication Date: 2017-08-07T17:39:35Z
• Authors: Chunhong Cheng; Zhijuan Wang; Ziyin Ren; Liya Zhi; Bin Yao; Chao Su; Liu Liu; Xia Li
• Keywords: Skp1; F-box protein; Protein Degradation

The phytohormone abscisic acid (ABA) is an essential part of the plant response to abiotic stressors such as drought. Upon perception ABA, pyrabactin resistance (PYR)/PYR1-like (PYL)/regulatory components ABA receptor (RCAR) proteins interact with co-receptor protein phosphatase type 2Cs permit activation Snf1-related kinase2 (SnRK2) kinases, which switch on signaling by phosphorylating various target proteins. Thus, SnRK2 kinases are central regulators signaling. However, mechanisms that regulate degradation remain elusive. Here, we show SnRK2.3 degradated 26S proteasome system and promotes its degradation. We found interacts AtPP2-B11 directly. F-box a SKP1/Cullin/F-box E3 ubiquitin ligase complex negatively regulates responses specifically promoting SnRK2.3. was induced knockdown expression markedly increased sensitivity plants during seed germination postgerminative development. Overexpression does not affect sensitivity, but inhibits hypersensitive phenotypes overexpression lines. These results reveal novel mechanism through degrades attenuate stress in Arabidopsis.