The CPEB translational regulator, Orb, functions together with Par proteins to polarize the Drosophila oocyte

• DOI: 10.1371/journal.pgen.1008012
• Publication Date: 2019-03-13T17:45:40Z
• Authors: Justinn Barr; Sofia Charania; Rudolf Gilmutdinov; Konstantin Yakovlev; Yulii Shidlovskii; Paul Schedl
• Keywords: oskar; Drosophila embryogenesis; Krüppel

orb is a founding member of the CPEB family translational regulators and required at multiple steps during Drosophila oogenesis. Previous studies showed that mid-oogenesis for translation posterior/germline determinant oskar mRNA dorsal-ventral gurken mRNA. Here, we report also functions upstream these axes determinants in polarization microtubule network (MT). Prior to activation, oocyte MT repolarized. The organizing center posterior disassembled, new established anterior. Repolarization depends upon cross-regulatory interactions between anterior (apical) (basal) Par proteins. We show repolarization requires needed proper functioning interacts genetically with aPKC cdc42 egg chambers compromised activity, Par-1 protein are mislocalized. Moreover, like cdc42-, defects localization appear be connected abnormalities cortical actin cytoskeleton. These disrupt spectraplakin Shot minus-end binding Patronin. two proteins play critical role network.