A secretion-enhancing cis regulatory targeting element (SECReTE) involved in mRNA localization and protein synthesis

• DOI: 10.1371/journal.pgen.1008248
• Publication Date: 2019-07-01T17:25:57Z
• Authors: Osnat Cohen-Zontag; Camila Baez; Lisha Qiu Jin Lim; Tsviya Olender; Dvir Schirman; Dvir Dahary; Yitzhak Pilpel; Jeffrey E. Gerst
• Keywords: Secretory protein

The localization of mRNAs encoding secreted/membrane proteins (mSMPs) to the endoplasmic reticulum (ER) likely facilitates co-translational translocation secreted proteins. However, studies have shown that mSMP recruitment ER in eukaryotes can occur a manner is independent ribosome, translational control, and signal recognition particle, although mechanism remains largely unknown. Here, we identify cis-acting RNA sequence motif enhances appears increase mRNA stability, both synthesis secretion secretome Termed SECReTE, for secretion-enhancing cis regulatory targeting element, this enriched translated on inner membrane prokaryotes. SECReTE consists ≥10 nucleotide triplet repeats with pyrimidine (C/U) every third base (i.e. NNY, where N = any nucleotide, Y pyrimidine) be present untranslated as well coding regions mRNA. Synonymous mutations elevate count given (e.g. SUC2, HSP150, CCW12) lead an protein yeast, while reduction led less physiological defects. Moreover, addition 3'UTR exogenously expressed GFP) its increased from yeast cells. Thus, constitutes novel ER-localized translation secretion.