GLP-1 Notch—LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1

• DOI: 10.1371/journal.pgen.1008650
• Publication Date: 2020-03-20T17:53:54Z
• Authors: Jian Chen; Ariz Mohammad; Nanette Pazdernik; Huiyan Huang; Beth Bowman; Eric Tycksen; Tim Schedl
• Keywords: Cell fate determination

Stem cell systems are essential for the development and maintenance of polarized tissues. Intercellular signaling pathways control stem systems, where niche cells signal to maintain fate/self-renewal inhibit differentiation. In C. elegans germline, GLP-1 Notch specifies fate, employing sequence-specific DNA binding protein LAG-1 implement transcriptional response. We undertook a comprehensive genome-wide approach identify targets signaling. expected primary response target genes be evident at intersection identified as directly bound by LAG-1, from ChIP-seq experiments, with requiring RNA accumulation, RNA-seq analysis. Furthermore, we performed time-course transcriptomics analysis following auxin inducible degradation distinguish between whose level was or secondary Surprisingly, only lst-1 sygl-1, two known in fulfilled these criteria, indicating that may sole germline protein-coding mediating fate. addition, three were based on their timing loss lack peak glp-1 dependent mRNA accumulation could explained requirement sygl-1 activity. Moreover, our also suggests function can account FBF-2, which promotes fate and, part, spatial restriction elevated region.