Resistance to pirimiphos-methyl in West African Anopheles is spreading via duplication and introgression of the Ace1 locus

• DOI: 10.1371/journal.pgen.1009253
• Publication Date: 2021-01-21T18:25:27Z
• Authors: Xavier Grau-Bové; Eric Lucas; Dimitra Pipini; Emily Rippon; Arjèn E. van ‘t Hof; Edi Constant; Samuel Dadzie; Alexander Egyir-Y...; John Essandoh; Joseph Chabi; Luc Djogbénou; Nicholas J. Harding; Alistair Miles; Dominic Kwiatkowski; Martin J. Donnelly; David Weetman
• Keywords: Insecticides; африканские комары; DNA Copy Number Variations; Mosquito Vectors; QH426-470; Genetic Introgression; Insecticide Resistance; пиримифос-метил; 03 medical and health sciences; [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases; Genes, Duplicate; qx_600; Anopheles; Genetics; Animals; Humans; 0303 health sciences; malaria vectors; genomics; insecticide resistance; anopheles gambiae; anopheles coluzzii; wa_240; Organothiophosphorus Compounds; Malaria; 3. Good health; Africa, Western; qx_510; [SDE]Environmental Sciences; Acetylcholinesterase; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; qx_515; Research Article

Vector population control using insecticides is a key element of current strategies to prevent malaria transmission in Africa. The introduction of effective insecticides, such as the organophosphate pirimiphos-methyl, is essential to overcome the recurrent emergence of resistance driven by the highly diverse Anopheles genomes. Here, we use a population genomic approach to investigate the basis of pirimiphos-methyl resistance in the major malaria vectors Anopheles gambiae and A. coluzzii. A combination of copy number variation and a single non-synonymous substitution in the acetylcholinesterase gene, Ace1, provides the key resistance diagnostic in an A. coluzzii population from Côte d’Ivoire that we used for sequence-based association mapping, with replication in other West African populations. The Ace1 substitution and duplications occur on a unique resistance haplotype that evolved in A. gambiae and introgressed into A. coluzzii, and is now common in West Africa primarily due to selection imposed by other organophosphate or carbamate insecticides. Our findings highlight the predictive value of this complex resistance haplotype for phenotypic resistance and clarify its evolutionary history, providing tools to for molecular surveillance of the current and future effectiveness of pirimiphos-methyl based interventions.