F-actin coordinates spindle morphology and function in Drosophila meiosis

• DOI: 10.1371/journal.pgen.1011111
• Publication Date: 2024-01-11T18:38:46Z
• Authors: Benjamin W. Wood; Xingzhu Shi; Timothy T. Weil
• Keywords: Spindle pole body; Actin remodeling; Meiosis II

Meiosis is a highly conserved feature of sexual reproduction that ensures germ cells have the correct number chromosomes prior to fertilization. A subset microtubules, known as spindle, are essential for accurate chromosome segregation during meiosis. Building evidence in mammalian systems has recently highlighted unexpected requirement actin cytoskeleton segregation; network spindle filaments appear regulate many aspects this process. Here we show Drosophila oocytes also population appears formation microtubule and chromosomal movements throughout We demonstrate genetic pharmacological disruption significant impact on morphology, dynamics, alignment maturation metaphase-anaphase transition. further reveal role calcium maintaining actin. Together, our data highlights potential conservation morphology mechanism