High Cyclin E Staining Index in Blastemal, Stromal or Epithelial Cells Is Correlated with Tumor Aggressiveness in Patients with Nephroblastoma

• DOI: 10.1371/journal.pone.0002216
• Publication Date: 2008-05-20T22:19:39Z
• Authors: Dominique Berrebi; Julie Leclerc; Gudrun Schleierma...; Isabelle Zaccaria; Liliane Boccon-Gibod; Monique Fabre; Francis Jaubert; Alaa El Ghoneimi; Cécile Jeanpierre; Michel Peuchmaur
• Keywords: Cyclin B1; Ki-67; Cyclin E; Cyclin A; Proliferation index

Identifying among nephroblastoma those with a high propensity for distant metastases using cell cycle markers: cyclin E as regulator of progression through the and Ki-67 tumor proliferation marker, since both are often deregulated in many human malignancies.A staining index (SI) was obtained by immunohistochemistry anti-cyclin anti-Ki-67 antibodies paraffin sections 54 postchemotherapy including 42 without metastasis 12 metastases. Median SI were 46% 33% blastemal cells, 30% 10% stromal 37% 29.5% epithelial cells. The highest values found anaplastic nephroblastoma. A correlation between component component. Univariate analysis showed prognostic significance cells (p = 0.03, p 0.01 0.002, respectively) well blastema (p<10(-4)). most striking data that discriminated patients intermediate-risk nephroblastoma.Our findings show all components is correlated aggressiveness metastases, assessment its expression may have value categorization