H5N1 Vaccine-Specific B Cell Responses in Ferrets Primed with Live Attenuated Seasonal Influenza Vaccines

• DOI: 10.1371/journal.pone.0004436
• Publication Date: 2009-02-11T16:17:11Z
• Authors: Xing Cheng; Michael Eisenbraun; Qi Xu; Helen Zhou; Deepali Kulkarni; Kanta Subbarao; George Kemble; Hong Jin
• Keywords: Attenuated vaccine; ELISPOT

Live attenuated influenza H5N1 vaccines have been produced and evaluated in mice ferrets that were never exposed to A virus infection (Suguitan et al., Plos Medicine, e360:1541, 2006). However, the preexisting heterosubtypic immunity on live vaccine induced immune response has not evaluated.Primary recall B cell responses viruses examined using a sensitive antigen-specific ELISpot assay investigate effect of development H5N1-specific ferrets. A/Hong Kong/213/03 A/Vietnam/1203/04 measurable H5-specific IgM IgG secreting cells after intranasal vaccination. detected significantly earlier at greater frequency inoculation previously primed with trivalent (H1N1, H3N2 B) vaccine. Priming studies further revealed more rapid H5 resulted from cross-reactive hemagglutinin H1 protein. Moreover, vaccination H1N1 was able induce protective capable limiting replication level comparable prior without affecting antibody response.The findings indicate previous seasonal may accelerate onset by an ca be beneficial for pandemic preparedness.