Roles of ES Cell-Derived Gliogenic Neural Stem/Progenitor Cells in Functional Recovery after Spinal Cord Injury

• DOI: 10.1371/journal.pone.0007706
• Publication Date: 2009-11-05T23:49:48Z
• Authors: Gentaro Kumagai; Yohei Okada; Junichi Yamane; Narihito Nagoshi; Kazuya Kitamura; Masahiko Mukaino; Osahiko Tsuji; Kanehiro Fujiyoshi; Hiroyuki Katoh; Seiji Okada; Shinsuke Shibata; Yumi Matsuzaki; Satoshi Toh; Yoshiaki Toyama; Masaya Nakamura; Hideyuki Okano
• Keywords: Neurosphere; Remyelination; Neuroglia

Transplantation of neural stem/progenitor cells (NS/PCs) following the sub-acute phase spinal cord injury (SCI) has been shown to promote functional recovery in rodent models. However, types most effective for treating SCI have not clarified. Taking advantage our recently established neurosphere-based culture system ES cell-derived NS/PCs, which primary neurospheres (PNS) and passaged secondary (SNS) exhibit neurogenic gliogenic potentials, respectively, here we examined distinct effects transplanting NS/PCs on a mouse model SCI. PNS SNS transplanted 9 days after contusive at Th10 level exhibited differentiation tendencies, similar those seen vitro. Interestingly, transplantation SNS, but PNS, promoted axonal growth, remyelination, angiogenesis, resulted significant locomotor These findings suggest that are promoting from SCI, provide essential insight into mechanisms through cellular leads improvement