Spheres Derived from Lung Adenocarcinoma Pleural Effusions: Molecular Characterization and Tumor Engraftment

• DOI: 10.1371/journal.pone.0021320
• Publication Date: 2011-07-18T20:50:40Z
• Authors: Rita Mancini; Enrico Giarnieri; Claudia De Vitis; Donatella Malanga; Giuseppe Roscilli; Alessia Noto; Emanuele Marra; Carmelo Laudanna; Pietro Zoppoli; Pasquale De Luca; Andrea Affuso; Luigi Ruco; Arianna Di Napoli; Giuseppe Mesiti; Luigi Aurisicchio; Alberto Ricci; Salvatore Mariotta; Lara Pisani; Claudio Andreetti; Giuseppe Viglietto; Erino A. Rendina; Maria Rosaria Gio...; Gennaro Ciliberto
• Keywords: Male; 0301 basic medicine; Lung Neoplasms; Science; Adenocarcinoma of Lung; Cell Separation; Mice, SCID; Adenocarcinoma; Immunophenotyping; Mice; 03 medical and health sciences; Biomarkers, Tumor; Cell Adhesion; Animals; Humans; Aged; Aged, 80 and over; Q; R; Computational Biology; Aldehyde Dehydrogenase; Immunohistochemistry; Adenocarcinoma; Adenocarcinoma of Lung; Aged; Aged, 80 and over; Aldehyde Dehydrogenase; Animals; Biomarkers, Tumor; Cell Adhesion; Cell Separation; Computational Biology; Female; Gene Expression Regulation, Neoplastic; Genes, Neoplasm; Humans; Immunohistochemistry; Immunophenotyping; Lung Neoplasms; Male; Mice; Mice, SCID; Middle Aged; Neoplastic Stem Cells; Pleural Effusion, Malignant; Reverse Transcriptase Polymerase Chain Reaction; Spheroids, Cellular; Tumor Cells, Cultured; Xenograft Model Antitumor Assays; 3. Good health; Gene Expression Regulation, Neoplastic; Medicine; Female; Research Article; Genes, Neoplasm

Malignant pleural effusions (MPEs) could represent an excellent source to culture a wide variety of cancer cells from different donors. In this study, we set up conditions for deriving MPEs several patients affected by the most frequent form lung cancer, namely subset non small cell cancers (NSCLC) classified as Lung Adenocarcinomas (AdenoCa) which account approximately 40% cases. AdenoCa malignant gave rise in vitro cultures both adherent and/or spheroid almost all cases analyzed. We characterized greater detail two samples showed efficient propagation vitro. these also compared gene profiles vs and identified differentially expressed genes. Finally achieved tumor engraftment recipient NOD/SCID mice, upon inoculation number cells, thus suggesting indirectly presence initiating cells.