The Translation Elongation Factor eEF-1Bβ1 Is Involved in Cell Wall Biosynthesis and Plant Development in Arabidopsis thaliana

• DOI: 10.1371/journal.pone.0030425
• Publication Date: 2012-01-17T21:50:18Z
• Authors: Zakir Hossain; Lisa Amyot; Brian McGarvey; Margaret Gruber; Jinwook Jung; Abdelali Hannoufa
• Keywords: Wild type

The eukaryotic translation elongation factor eEF-1Bβ1 (EF1Bβ) is a guanine nucleotide exchange that plays an important role in elongation. In this study, we show the EF1Bβ protein localized plasma membrane and cytoplasm, transcripts should be expressed most tissue types seedlings. Sectioning of inflorescence stem revealed predominantly localizes to xylem vessels interfascicular cambium. gene silencing efβ caused dwarf phenotype with 38% 20% reduction total lignin crystalline cellulose, respectively. This loss-of-function mutant also had lower S/G monomer ratio relative wild type plants, but no changes were detected gain-of-function transformed gene. Histochemical analysis showed reduced vascular apparatus, including smaller mutant. Over-expression eli1 background restored WT abolished ectopic deposition as well cell expansion defects Taken together, these data strongly suggest for plant development wall formation Arabidopsis.