Whole Transcriptome Analysis of the Effects of Type I Diabetes on Mouse Oocytes

• DOI: 10.1371/journal.pone.0041981
• Publication Date: 2012-07-24T21:11:53Z
• Authors: Jun-Yu Ma; Mo Li; Zhao-Jia Ge; Yibo Luo; Xiang-Hong Ou; Shuhui Song; Dongmei Tian; Jin Yang; Bing Zhang; Ying-Chun Ou-Yang; Yi Hou; Zhonghua Liu; Heide Schatten; Qing-Yuan Sun
• Keywords: Embryo quality

In mouse ovarian follicles, granulosa cells but not oocytes take up glucose to provide the oocyte with nourishments for energy metabolism. Diabetes-induced hyperglycemia or absorption inefficiency consistently causes cell apoptosis and further exerts a series of negative impacts on including reduced meiosis resumption rate, low quality preimplantation embryo degeneration. Here we compared transcriptome from genetically derived NOD diabetic mice chemically induced STZ that corresponding normal mice. Differentially expressed genes were extracted two models. Gene set enrichment analysis showed associated metabolic developmental processes differentially in both models diabetes. addition, diabetes also affected expression ovulation, cycle progression, development. Notably, Dnmt1 was significantly down-regulated, Mbd3 up-regulated oocytes. Our data only revealed mechanisms by which affects development, linked epigenetic hereditary factors disorders germ cells.