Identification and Expression of Nine Oak Aquaporin Genes in the Primary Root Axis of Two Oak Species, Quercus petraea and Quercus robur

• DOI: 10.1371/journal.pone.0051838
• Publication Date: 2012-12-17T21:56:43Z
• Authors: Claire Rasheed-De...; Claire Parent; Michèle Crèvecoeur; Julien Parelle; Fabienne Tatin-Froux; Grégoire Le Provost; Nicolas Capelli
• Keywords: Quercus petraea; Quercus robur; Water Transport

Aquaporins (AQPs) belong to the Major Intrinsic Protein family that conducts water and other small solutes across biological membranes. This study aimed identify characterize AQP genes in primary root axis of two oak species, Quercus petraea robur. Nine putative were cloned, their expression was profiled different developmental zones by real-time PCR. A detailed examination predicted amino acid sequences subsequent phylogenetic analysis showed isolated AQPs could be divided into subfamilies, which included six plasma membrane intrinsic proteins (PIPs) three tonoplast (TIPs). We characterized anatomical features roots defined zones: immature, transition mature zones. Expression performed according these stages. Our results PIP2;3 TIP1 significantly higher compared with robur However, PIP2;1 TIP2;1 found differentially expressed zone species. Of nine identified analyzed, we highlighted four might facilitate a deeper understanding how closely related tree species adapted environments.