The Apoptotic Effects of Toosendanin Are Partially Mediated by Activation of Deoxycytidine Kinase in HL-60 Cells

• DOI: 10.1371/journal.pone.0052536
• Publication Date: 2012-12-27T23:53:37Z
• Authors: Jianming Ju; Zhichao Qi; Xueting Cai; Peng Cao; Yan Huang; Shuzhen Wang; Nan Liu; Yijun Chen
• Keywords: Deoxycytidine kinase

Triterpenoid toosendanin (TSN) exhibits potent cytotoxic activity through inducing apoptosis in a variety of cancer cell lines. However, the target and mechanism apoptotic effects by TSN remain unknown. In this study, we captured specific binding protein HL-60 cells serial affinity chromatography further identified it as deoxycytidine kinase (dCK). Combination direct activation dCK inhibition TSN-induced inhibitor confirmed that is for partially responsible cells. Moreover, was result conformational change, rather than auto-phosphorylation. Our results imply that, addition to dATP increase tumor cells, may also involve regulation.