Rapid and Serial Quantification of Adhesion Forces of Yeast and Mammalian Cells

• DOI: 10.1371/journal.pone.0052712
• Publication Date: 2012-12-20T22:41:45Z
• Authors: Eva Potthoff; Orane Guillaume-G...; Dario Ossola; Jérôme Polesel-Maris; Salomé LeibundGut...; Tomaso Zambelli; Julia A. Vorholt
• Keywords: Force Spectroscopy

Cell adhesion to surfaces represents the basis for niche colonization and survival. Here we establish serial quantification of forces different cell types using a single probe. The pace single-cell force-spectroscopy was accelerated up 200 yeast 20 mammalian cells per probe when replacing conventional trapping cantilever chemistry atomic force microscopy by underpressure immobilization with fluidic (FluidFM). In consequence, statistically relevant data could be recorded in rapid manner, spectrum examinable enlarged, physiology preserved until approached spectroscopy. Adhesion Candida albicans increased from below 4 16 nN at 37°C on hydrophobic surfaces, whereas Δhgc1-mutant showed consistently nN. Monitoring revealed mean 600 HeLa fibronectin were one order magnitude higher than those observed HEK cells.