Cloning and Characterization of the Human USP22 Gene Promoter

• DOI: 10.1371/journal.pone.0052716
• Publication Date: 2012-12-26T17:09:54Z
• Authors: Jianjun Xiong; Xiangxin Che; Xueqin Li; Huan Yu; Zhen Gong; Weidong Li
• Keywords: Transcription; Sp1 transcription factor

Ubiquitin-specific processing enzyme 22 (USP22) plays a direct role in regulating cell cycle, and its overexpression has been reported to be involved tumor progression. However, little is known about the regulation of USP22 transcription. In this study, we cloned characterized human promoter. Using 5' RACE (rapid amplification cDNA ends) analysis, transcriptional initiation site was identified. Promoter deletion analysis showed that sequence between -210 -7 contains basal promoter for fibroblast cells. Surprisingly, mutations putative Sp1 binding immediately upstream start (-13 -7) resulted significant induction activity. Further study revealed binds normal cells, treatment with inhibitor mithramycin A led marked increase transcript levels. Forced expression exogenous repressed activity HeLa contrast, knockdown enhanced mRNA These data suggest crucial regulator