Impact of CD68/(CD3+CD20) Ratio at the Invasive Front of Primary Tumors on Distant Metastasis Development in Breast Cancer

• DOI: 10.1371/journal.pone.0052796
• Publication Date: 2012-12-26T22:07:17Z
• Authors: Noemí Eiró; Iván Pidal; Belen Fernandez-G...; Sara Junquera; Maria L. Lamelas; José M. del Casar; Luis O. González; Alfonso López-Muñiz; Francisco J. Vizoso
• Keywords: CD68

Tumors are infiltrated by macrophages, T and B-lymphocytes, which may favor tumor development promoting angiogenesis, growth invasion. The aim of this study was to investigate the clinical relevance relative amount macrophages (CD68+), T-cells (CD3+) B-cells (CD20+) at invasive front breast carcinomas, expression matrix metalloproteases (MMPs) their inhibitors (TIMPs) either or center. We performed an immunohistochemical counting CD3, CD20 CD68 positive cells front, in 102 carcinomas. Also, tissue sections were stained with MMP-2, -9, -11, -14 TIMP-2 antibodies, immunoreactivity location, percentage reactive area intensity determined results showed that increased count CD68/(CD3+CD20) ratio directly associated both MMP-11 mononuclear inflammatory center (p = 0.041 p 0.025 for 0.001 0.045 ratio, respectively TIMP-2). In addition, a high (>0.05) higher probability shortened relapse-free survival. Multivariate analysis revealed independent factor distant survival (RR: 2.54, CI: (1.23–5.24), p<0.01). Therefore, could be used as important prognostic marker.