A Novel Approach to Enhancing Ganoderic Acid Production by Ganoderma lucidum Using Apoptosis Induction

• DOI: 10.1371/journal.pone.0053616
• Publication Date: 2013-01-10T17:16:30Z
• Authors: Bang-Jau You; Miin-Huey Lee; Ni Tien; Meng-Shiou Lee; Hui-Chuan Hsieh; Lin-Hsien Tseng; Yu-Lin Chung; Hong-Zin Lee
• Keywords: Squalene; Terminal deoxynucleotidyl transferase

Ganoderma lucidum is one of most widely used herbal medicine and functional food in Asia, ganoderic acids (GAs) are its active ingredients. Regulation GA biosynthesis enhancing production critical to using G. as a medicine. However, regulation by various signaling remains poorly understood. This study investigated the role apoptosis on presented novel approach, namely induction, increasing production. Aspirin was able induce cell lucidum, which identified terminal deoxynucleotidyl transferase mediated dUPT nick end labeling assay positive staining condensed nuclear morphology. The maximum induction lanosta-7,9(11), 24-trien-3α-01-26-oic acid (ganoderic 24, GA24) total aspirin were 2.7-fold 2.8-fold, respectively, after 1 day. Significantly lower levels 24 GAs obtained regular fungal culture for 1.5 months. ROS accumulation phosphorylation Hog-1 kinase, putative homolog MAPK p38 mammals, occurred treatment indicating that both factors may be involved biosynthetic regulation. also reduced expression squalene synthase lanosterol coding genes, suggesting these genes not induction. To best our knowledge, this first report showing linked provides new approach secondary metabolite fungi.