The Effect of Heparin-VEGF Multilayer on the Biocompatibility of Decellularized Aortic Valve with Platelet and Endothelial Progenitor Cells

• DOI: 10.1371/journal.pone.0054622
• Publication Date: 2013-01-24T22:43:08Z
• Authors: Xiaofeng Ye; Haozhe Wang; Jingxin Zhou; Haiqing Li; Jun Liu; Zhe Wang; Anqing Chen; Qiang Zhao
• Keywords: Decellularization; Biocompatibility

The application of polyelectrolyte multilayer films is a new, versatile approach to surface modification decellularized tissue, which has the potential greatly enhance functionality engineered tissue constructs derived from organs. In present study, we test hypothesis that Heparin- vascular endothelial growth factor (VEGF) film can not only act as an antithrombotic coating reagent, but also induce proliferation progenitor cells (EPCs) on aortic heart valve. SEM demonstrated adhesion and geometric deformation platelets. quantitative assay platelet activation was determined by measuring production soluble P-selectin. Binding subsequent release heparin VEGF valve leaflets were assessed qualitatively laser confocal scanning microscopy quantitatively ELISA methods. Human blood EPCs cultured modified valvular scaffolds assessed. results showed Heparin-VEGF improved haemocompatibility with respect substantial reduction adhesion. Release at physiological conditions sustained over 5 days. vitro biological tests achieved better adhesion, migration coatings film. Combined, these indicate could be used cover porcine decrease while exhibiting excellent biocompatibility.