Cell Differentiation of Pluripotent Tissue Sheets Immobilized on Supported Membranes Displaying Cadherin-11

• DOI: 10.1371/journal.pone.0054749
• Publication Date: 2013-02-12T22:15:03Z
• Authors: Alexander Körner; Christina Deichmann; Fernanda F. Rossetti; Almut Köhler; Oleg V. Konovalov; Doris Wedlich; Motomu Tanaka
• Keywords: Pluripotent Stem Cells; 0301 basic medicine; 570; Embryo, Nonmammalian; [SDV]Life Sciences [q-bio]; Science; Xenopus Proteins; Xenopus laevis; 03 medical and health sciences; Cell Adhesion; Animals; Histidine; info:eu-repo/classification/ddc/570; biology; Q; Cell Membrane; R; Cell Differentiation; Blastula; Cells, Immobilized; Cadherins; Life sciences; Medicine; ddc:570; Research Article

Investigating cohesive tissue sheets in controlled cultures still poses a challenge since the complex intercellular interactions are difficult to mimic vitro models. We used supported lipid membranes functionalized by adhesive part of extracellular domain cell adhesion molecule cadherin-11 for immobilization pluripotent sheets, animal cap isolated from Xenopus laevis blastula stage embryos. Cadherin-11 was bound via histidine tag with chelator head groups. In first step, quantitative functionalization confirmed quartz crystal microbalance and high energy specular X-ray reflectivity. next induced neural crest fate were cultured on cadherin-11. The cells within significantly dependent changes lateral densities formation filopodia lamellipodia verified viability sustainability culture over several hours. expression transcription factor slug externally demonstrated applicability displaying molecules differentiation sheets.