miR-153 Regulates SNAP-25, Synaptic Transmission, and Neuronal Development

• DOI: 10.1371/journal.pone.0057080
• Publication Date: 2013-02-25T22:14:20Z
• Authors: Chunyao Wei; Elizabeth J. That...; Abigail F. Olena; Diana J. Cha; Ana L. Perdigoto; Andrew F. Marshall; Bruce D. Carter; Kendal Broadie; James G. Patton
• Keywords: Snap

SNAP-25 is a core component of the trimeric SNARE complex mediating vesicle exocytosis during membrane addition for neuronal growth, neuropeptide/growth factor secretion, and neurotransmitter release synaptic transmission. Here, we report novel microRNA mechanism regulation controlling motor neuron development, neurosecretion, activity, movement in zebrafish. Loss miR-153 causes overexpression consequent hyperactive early zebrafish embryos. Conversely, down resulting near complete paralysis, mimicking effects treatment with Botulinum neurotoxin. miR-153-dependent changes activity at neuromuscular junction are consistent observed defects. Underlying defects, perturbation function dramatic developmental patterning branching. Together, our results indicate that precise control expression by critically important proper as well neurotransmission.