Mapping the Interactions of Dengue Virus NS1 Protein with Human Liver Proteins Using a Yeast Two-Hybrid System: Identification of C1q as an Interacting Partner

• DOI: 10.1371/journal.pone.0057514
• Publication Date: 2013-03-14T23:50:31Z
• Authors: Emiliana M. Silva; Jonas N. Conde; Diego Allonso; Mauricio L. Nogueira; Ronaldo Mohana-Bo...
• Keywords: Hemopexin; Pathogenesis; C4A

Dengue constitutes a global health concern. The clinical manifestation of this disease varies from mild febrile illness to severe hemorrhage and/or fatal hypovolemic shock. Flavivirus nonstructural protein 1 (NS1) is secreted glycoprotein that displayed on the surface infected cells but absent in viral particles. NS1 accumulates at high levels plasma dengue virus (DENV)-infected patients, and previous reports highlight its involvement immune evasion, severity, liver dysfunction pathogenesis. In present study, we performed yeast two-hybrid screen search for DENV2 NS1-interacting partners using human cDNA library. We identified fifty genes, including complement component (C1q), which was confirmed by coimmunoprecipitation, ELISA immunofluorescence assays, revealing first time direct binding NS1. Furthermore, majority genes encode proteins are into most these classified as acute-phase (APPs), such plasminogen, haptoglobin, hemopexin, α-2-HS-glycoprotein, retinol 4, transferrin, C4. results presented here confirm interaction DENV with key system suggest role pathogenesis infection.