Genome-Wide RNAi Screening Identifies Genes Inhibiting the Migration of Glioblastoma Cells

• DOI: 10.1371/journal.pone.0061915
• Publication Date: 2013-04-12T21:06:21Z
• Authors: Jian Yang; Jing Fan; Ying Li; Fuhai Li; Peikai Chen; Yubo Fan; Xiaofeng Xia; Stephen T. Wong
• Keywords: Science; Mice; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Animals; Humans; RNA, Small Interfering; 0303 health sciences; Cell Death; Brain Neoplasms; Genome, Human; Q; R; Reproducibility of Results; Survival Analysis; 3. Good health; Gene Expression Regulation, Neoplastic; Treatment Outcome; Gene Knockdown Techniques; Medicine; RNA Interference; Glioblastoma; Research Article; Genes, Neoplasm

Glioblastoma Multiforme (GBM) cells are highly invasive, infiltrating into the surrounding normal brain tissue, making it impossible to completely eradicate GBM tumors by surgery or radiation. Increasing evidence also shows that these migratory resistant cytotoxic reagents, but decreasing their capability can re-sensitize them chemotherapy. These evidences suggest cell population may serve as a better therapeutic target for more effective treatment of GBM. In order understand regulatory mechanism underlying motile phenotype, we carried out genome-wide RNAi screen genes inhibiting migration cells. The screening identified total twenty-five primary hits; seven were confirmed secondary screening. Further study showed three genes, FLNA, KHSRP and HCFC1, functioned in vivo, knocking down caused multifocal tumor mouse model. Interestingly, two found be correlated with clinical outcome patients. have not been previously associated migration.