Peritoneal Fluid Reduces Angiogenesis-Related MicroRNA Expression in Cell Cultures of Endometrial and Endometriotic Tissues from Women with Endometriosis

• DOI: 10.1371/journal.pone.0062370
• Publication Date: 2013-04-19T17:06:09Z
• Authors: Aitana Braza-Boïls; Juan Gilabert-Est...; Luis A. Ramón; Juan Gilabert; Josep Marí-Alexandre; Melitina Chirivella; Francisco España; Amparo Estellés
• Keywords: Peritoneal fluid; Angiogenin; Plasminogen activator inhibitor-1

Endometriosis, defined as the presence of endometrium outside uterus, is one most frequent gynecological diseases. It has been suggested that modifications both endometrial and peritoneal factors could be implicated in this disease. Endometriosis a multifactorial disease which angiogenesis proteolysis are dysregulated. MicroRNAs (miRNAs) small non-coding RNAs regulate protein expression may main regulators angiogenesis. Our hypothesis fluid from women with endometriosis modify several miRNAs development. The objective study to evaluate influence endometriotic on six related angiogenesis, well angiogenic proteolytic cell cultures compared without endometriosis. Methods Endometrial cells were cultured treated control pools. We have studied (miR-16, -17-5p, -20a, -125a, -221, -222) by RT-PCR mRNA levels vascular endothelial growth factor-A, thrombospondin-1, urokinase plasminogen activator inhibitor-1 ELISA qRT-PCR respectively. Results Control pools induced significant reduction all cultures. Moreover, fluids increase VEGF-A, uPA PAI-1 levels. patients showed lower higher VEGF-A than controls. In conclusion, "in vitro" indicates modulates contribute disequilibrium observed