Presenilin Promotes Dietary Copper Uptake

• DOI: 10.1371/journal.pone.0062811
• Publication Date: 2013-05-08T01:25:28Z
• Authors: Adam Southon; Mark A. Greenough; George Ganio; Ashley I. Bush; Richard Burke; James Camakaris
• Keywords: 0301 basic medicine; 570; Superoxide Dismutase; Science; Q; R; Presenilins; 610; Biological Transport; Diet; 3. Good health; Oxidative Stress; 03 medical and health sciences; Drosophila melanogaster; Superoxide Dismutase-1; Gene Knockdown Techniques; Medicine; Animals; Insect Proteins; RNA Interference; Conserved Sequence; Copper; Research Article

Dietary copper is essential for multicellular organisms. Copper redox active and required as a cofactor enzymes such the antioxidant Superoxide Dismutase 1 (SOD1). dyshomeostasis has been implicated in Alzheimer's disease. Mutations presenilin genes encoding PS1 PS2 are major causes of early-onset familial efficient uptake mammalian systems. Here we demonstrate conserved role dietary fly Drosophila melanogaster. Ubiquitous RNA interference-mediated knockdown single (PSN) gene lethal. However, PSN midgut produces viable flies. These flies have reduced levels more tolerant to excess copper. Expression copper-responsive EYFP construct was also lower these larvae, indicative uptake. SOD activity by knockdown, were sensitive superoxide-inducing chemical paraquat. data support being needed gut so impacting on tolerance oxidative stress. results consistent with previous studies presenilins, supporting proteins mediating