Matrix Metalloproteinases Regulate the Formation of Dendritic Spine Head Protrusions during Chemically Induced Long-Term Potentiation

• DOI: 10.1371/journal.pone.0063314
• Publication Date: 2013-05-16T21:17:35Z
• Authors: Zsuzsanna Szepesi; Monika Bijata; Blazej Ruszczycki; Leszek Kaczmarek; Jakub Wlodarczyk
• Keywords: Dendritic filopodia

Dendritic spines are small membranous protrusions that extend from neuronal dendrites and harbor the majority of excitatory synapses. Increasing evidence has shown matrix metalloproteinases (MMPs), a family extracellularly acting Zn2+-dependent endopeptidases, able to rapidly modulate dendritic spine morphology. Spine head (SHPs) filopodia-like processes head, representing form postsynaptic structural remodeling in response altered activity. Herein, we show chemically induced long-term potentiation (cLTP) dissociated hippocampal cultures upregulates MMP-9 activity controls formation SHPs. Blocking MMPs or microtubule dynamics abolishes emergence In addition, autoactive recombinant MMP-9, promotes SHPs organotypic slices. Furthermore, with gained α-amino-3-hydroxyl-5-methyl-4-isoxazole propionic acid (AMPA) receptors upon cLTP synaptic delivery AMPA was controlled by MMPs. The present results strongly imply is functionally involved control receptor distribution cLTP.