Adiponectin Increases Secretion of Rat Submandibular Gland via Adiponectin Receptors-Mediated AMPK Signaling

• DOI: 10.1371/journal.pone.0063878
• Publication Date: 2013-05-08T00:47:52Z
• Authors: Chong Ding; Li Li; Yun-Chao Su; Ruo-Lan Xiang; Xin Cong; Hong-Kui Yu; Sheng-Lin Li; Li-Ling Wu; Guang-Yan Yu
• Keywords: Adiponectin receptor 1; Paracellular transport

Adiponectin and adiponectin receptors (AdipoR1/2) are expressed in various tissues involved the regulation of multiple functions such as energy metabolism inflammatory responses. However, effect AdipoRs submandibular glands has not been fully evaluated. In present study, we found that mRNA protein both AdipoR1/2 were rat SMG-C6 cell line, evidenced by RT-PCR Western blot analysis. Immunofluorescence staining showed was diffused cytoplasm, while concentrated membrane acinar cells. Saliva flow significantly increased full length (fAd) or globular (gAd) perfusion isolated glands. 5-Aminoimidazole-4-carboxamide-1-4-ribofuranoside (AICAR), an adenosine monophosphate activated kinase (AMPK) activator, also saliva secretion. fAd, gAd, AICAR all average width apical tight junctions perfused glands, decreased transepithelial electrical resistance (TER) cells, suggesting promoted secretion modulating paracellular permeability. fAd gAd p-AMPK levels, AraA, AMPK antagonist, abolished fAd- gAd-induced changes secretion, junction ultrastructure, TER. Moreover, AdipoR1 AdipoR2 required for TER responses, from their inhibition following knockdown, co-knockdown RNA interference. Our results suggest a promoter salivary via activation AdipoRs, AMPK,