Improvement in Regional CBF by L-Serine Contributes to Its Neuroprotective Effect in Rats after Focal Cerebral Ischemia

• DOI: 10.1371/journal.pone.0067044
• Publication Date: 2013-06-25T21:20:30Z
• Authors: Tao-Jie Ren; Ren Qiang; Zheng-Lin Jiang; Guo-Hua Wang; Li Sun; Rui Jiang; Guang-Wei Zhao; Le-Yang Han
• Keywords: Brain ischemia

To investigate the mechanisms underlying neuroprotective effect of L-serine, permanent focal cerebral ischemia was induced by occlusion middle artery while monitoring blood flow (CBF). Rats were divided into control and L-serine-treated groups after occlusion. The neurological deficit score brain infarct volume assessed. Nissl staining used to quantify cortical injury. L-serine D-serine levels in ischemic cortex analyzed with high performance liquid chromatography. We found that treatment: 1) reduced score, neuron loss a dose-dependent manner; 2) improved CBF cortex, this inhibited presence apamin plus charybdotoxin alleviation both blocked; 3) increased amount inhibition conversion aminooxyacetic acid did not affect reduction L-serine. In conclusion, improvement regional may contribute its on brain, potentially through vasodilation which is mediated small- intermediate-conductance Ca2+-activated K+ channels vessel endothelium.