Neuropathic Pain in Rats with a Partial Sciatic Nerve Ligation Is Alleviated by Intravenous Injection of Monoclonal Antibody to High Mobility Group Box-1

• DOI: 10.1371/journal.pone.0073640
• Publication Date: 2013-08-21T21:04:24Z
• Authors: Yoki Nakamura; Norimitsu Morioka; Hiromi Abe; Fang Fang Zhang; Kazue Hisaoka-Nak...; Keyue Liu; Masahiro Nishibori; Yoshihiro Nakata
• Keywords: HMGB1; Peripheral nerve injury; Nerve Injury

High mobility group box-1 (HMGB1) is associated with the pathogenesis of inflammatory diseases. A previous study reported that intravenous injection anti-HMGB1 monoclonal antibody significantly attenuated brain edema in a rat model stroke, possibly by attenuating glial activation. Peripheral nerve injury leads to increased activity glia spinal cord dorsal horn. Thus, it possible could also be efficacious peripheral injury-induced pain. Following partial sciatic ligation (PSNL), rats were treated either or control IgG. Intravenous treatment (2 mg/kg) ameliorated PSNL-induced hind paw tactile hypersensitivity at 7, 14 and 21 days, but not 3 after ligation, whereas IgG had no effect on hypersensitivity. The expression HMGB1 protein horn was days PSNL; efficacy likely related presence protein. Also, translocation from nucleus cytosol occurred mainly neurons astrocytes microglia, indicating neuronal source HMGB1. Markers astrocyte (glial fibrillary acidic (GFAP)), microglia (ionized calcium binding adaptor molecule 1 (Iba1)) neuron (cFos) greatly ipsilateral side compared sham-operated PSNL. Anti-HMGB1 decreased cFos Iba1, GFAP. results demonstrate evokes synthesis release neurons, facilitating both which turn symptoms neuropathic targeting useful therapeutic strategy chronic