Isolation and Characterization of Carbendazim-degrading Rhodococcus erythropolis djl-11

• DOI: 10.1371/journal.pone.0074810
• Publication Date: 2013-10-01T18:44:31Z
• Authors: Xinjian Zhang; Yujie Huang; Paul R. Harvey; Hongmei Li; Yan Ren; Jishun Li; Jianing Wang; Hetong Yang
• Keywords: Enrichment culture

Carbendazim (methyl 1H-benzimidazol-2-yl carbamate) is one of the most widely used fungicides in agriculture worldwide, but has been reported to have adverse effects on animal health and ecosystem function. A highly efficient carbendazim-degrading bacterium (strain dj1-11) was isolated from carbendazim-contaminated soil samples via enrichment culture. Strain dj1-11 identified as Rhodococcus erythropolis based morphological, physiological biochemical characters, including sequence analysis 16S rRNA gene. In vitro degradation carbendazim (1000 mg·L−1) by minimal salts medium (MSM) efficient, with an average rate 333.33 mg·L−1·d−1 at 28°C. The optimal temperature range for MSM 25–30°C. Whilst strain capable metabolizing cabendazim sole source carbon nitrogen, significantly (P<0.05) increased addition 12.5 mM NH4NO3. Changes pH (4–9), substitution NH4NO3 organic substrates N C sources or replacing Mg2+ Mn2+, Zn2+ Fe2+ did not affect dj1-11. During process, liquid chromatography-mass spectrometry (LC-MS) detected metabolites 2-aminobenzimidazole 2-hydroxybenzimidazole. putative carbendazim-hydrolyzing esterase gene cloned chromosomal DNA djl-11 showed 99% homology mheI Nocardioides sp. SG-4G.