CD8+ Treg Cells Associated with Decreasing Disease Activity after Intravenous Methylprednisolone Pulse Therapy in Lupus Nephritis with Heavy Proteinuria

• DOI: 10.1371/journal.pone.0081344
• Publication Date: 2014-01-27T21:19:19Z
• Authors: Yi-Giien Tsai; Chia-Ying Lee; Tze-Yi Lin; Ching-Yuang Lin
• Keywords: Nephritis

We focus on the role of CD8+ Treg cell in Intravenous methyl-prednisolone (IVMP) pulse therapy forty patients with active Class III/IV childhood lupus nephritis (LN) heavy proteinuria. IVMP for five days. From peripheral blood mononuclear cells (PBMCs) and renal tissues, we saw definitely restoring both CD4+CD25+FoxP3+ CD8+CD25+Foxp3+ number plus greater expression intracellular IL-10 granzyme B CD8+FoxP3+ from PBMCs. IVMP-treated CD8+CD25+ directly suppressed CD4+ T proliferation induced CD4+CD45RO+ apoptosis. Histologically, CD4+FoxP3+ as well appeared tissue LN before by double immunohistochemical stain. increased 10 follow-up biopsy specimens after IVMP. Reverse correlation serum anti-C1q antibody FoxP3+ PBMNCs (r = −0.714, P<0.01). After IVMP, decrease accompanied increase cells. CD8+Treg reduced interferon-r response PBMCs to major peptide autoepitopes nucleosomes therapy; siRNA FoxP3 while decreasing CD8+CD25+Treg-induced Renal activity SLEDAI-2k was significantly higher than two weeks (P<0.01). return post-IVMP exert crucial immune modulatory effect control autoimmune LN. Trial Registration DMR97-IRB-259