Glycosylation of Immunoglobulin G: Role of Genetic and Epigenetic Influences

• DOI: 10.1371/journal.pone.0082558
• Publication Date: 2013-12-06T21:30:34Z
• Authors: Cristina Menni; Toma Keser; Massimo Mangino; Jordana T. Bell; Idil Erte; Irena Akmačić; Frano Vučković; Maja Pučić Baković; Olga Gornik; Mark I. McCarthy; Vlatka Zoldoš; Tim D. Spector; Gordan Lauc; Ana M. Valdes
• Keywords: Glycome; Epigenome; Genetic Association

ObjectiveTo determine the extent to which genetic and epigenetic factors contribute variations in glycosylation of immunoglobulin G (IgG) humans. Methods76 N-glycan traits circulating IgG were analyzed by UPLC 220 monozygotic 310 dizygotic twin pairs from TwinsUK. A classical study design was used derive additive genetic, common unique environmental components defining variance these traits. Epigenome-wide association analysis performed using Illumina 27k chip. Results51 76 glycan studied have an component (heritability, h2)≥ 0.5. In contrast, 12 had a low contribution (h2<0.35). We then tested for between methylation levels (P<2 x10-6). Among with heritability probe cg08392591 maps CpG island 5' ANKRD11 gene, p53 activator on chromosome 16. Probe cg26991199 SRSF10 gene involved regulation RNA splicing particularly mRNA precursors upon heat shock. those high we found cg13782134 (mapping NRN1L gene) cg16029957 mapping near QPCT be array-wide significant. The proportion associations significantly larger (P<0.005) among glycans (42%) than (6.2%). ConclusionsGlycome analyses might provide useful integration non-genetic further our understanding role both normal physiology disease.