T Cells Detect Intracellular DNA but Fail to Induce Type I IFN Responses: Implications for Restriction of HIV Replication

• DOI: 10.1371/journal.pone.0084513
• Publication Date: 2014-01-03T21:21:44Z
• Authors: Randi K. Berg; Stine H. Rahbek; Emil Kofod-Olsen; Christian K. Holm; Jesper Melchjorsen; David G. Jensen; Anne Louise Hansen; Louise B. Jørgensen; Lars Ostergaard; Martin Tolstrup; Carsten S. Larsen; Søren R. Paludan; Martin R. Jakobsen; Trine H. Mogensen
• Keywords: Permissiveness

HIV infects key cell types of the immune system, most notably macrophages and CD4+ T cells. Whereas represent an important viral reservoir, activated cells are permissive supporting high levels replication. In recent years, it has been appreciated that innate system plays role in controlling replication, e.g. via interferon (IFN)-inducible restriction factors. Moreover, responses involved driving chronic activation pathogenesis progressive immunodeficiency. Several pattern recognition receptors detecting have reported, including Toll-like receptor 7 Retinoic-inducible gene-I, which detects RNA. Here we report human primary fail to induce strong IFN responses, despite fact this type does express molecules DNA signaling pathways. We demonstrate sensor IFI16 migrates sites foreign localization cytoplasm recruits stimulator genes Tank-binding kinase, but not result expression IFN-stimulated genes. Importantly, show cytosolic fails affect However, exogenous treatment with I capacity suppress Our data suggest existence impaired machinery cells, may prevent from activating cell-autonomous anti-HIV responses. This phenomenon could contribute permissiveness for HIV-1.