Identification and Characterization of MicroRNAs in Ovary and Testis of Nile Tilapia (Oreochromis niloticus) by Using Solexa Sequencing Technology

• DOI: 10.1371/journal.pone.0086821
• Publication Date: 2014-01-23T23:47:11Z
• Authors: Jun Xiao; Huan Zhong; Yi Zhou; Fan Yu; Yun Gao; Yongju Luo; Zhanyang Tang; Zhongbao Guo; Enyan Guo; Xi Gan; Ming Zhang; Yaping Zhang
• Keywords: Nile tilapia

MicroRNAs (miRNAs) are endogenous non-coding small RNAs which play important roles in the regulation of gene expression by cleaving or inhibiting translation target transcripts. Thereinto, some specific miRNAs show regulatory activities gonad development via translational control. In order to further understand role miRNA-mediated posttranscriptional Nile tilapia (Oreochromis niloticus) ovary and testis, two RNA libraries were sequenced Solexa deep sequencing methods. A total 9,731,431 8,880,497 raw reads, representing 5,407,800 4,396,281 unique sequences obtained from sexually mature ovaries testes, respectively. After comparing with Rfam database, 1,432,210 reads 984,146 testes matched genome sequence tilapia. Bioinformatic analysis identified 764 miRNA, 209 miRNA-5p 202 miRNA-3p found libraries, 525 known both expressed testis Comparison profiles miR-727, miR-129 miR-29 families highly ovary. Additionally, miR-132, miR-212, miR-33a miR-135b families, showed significant higher compared that Furthermore, patterns analyzed different developmental stages gonad. The result observed during addition, identification characterization differentially provides information on miRNA ovarian testicular function. This data will be helpful facilitate studies teleosts reproduction.