Accelerated Repair and Reduced Mutagenicity of DNA Damage Induced by Cigarette Smoke in Human Bronchial Cells Transfected with E.coli Formamidopyrimidine DNA Glycosylase

• DOI: 10.1371/journal.pone.0087984
• Publication Date: 2014-01-31T23:01:49Z
• Authors: Mara Foresta; Alberto Izzotti; Sebastiano La Mae...; Rosanna Micale; Alessandro Poggi; Donatella Vecchio; Guido Frosina
• Keywords: 0301 basic medicine; DNA Repair; Science; Escherichia coli Proteins; Q; R; Bronchi; Middle Aged; Transfection; Recombinant Proteins; 3. Good health; 03 medical and health sciences; DNA-Formamidopyrimidine Glycosylase; Cell Line, Tumor; Escherichia coli; Medicine; Humans; Female; Tobacco Smoke Pollution; Research Article; DNA Damage

Cigarette smoke (CS) is associated to a number of pathologies including lung cancer. Its mutagenic and carcinogenic effects are partially linked the presence reactive oxygen species polycyclic aromatic hydrocarbons (PAH) inducing DNA damage. The bacterial repair enzyme formamidopyrimidine glycosylase (FPG) repairs both oxidized bases different types bulky adducts. We investigated in vitro whether FPG expression may enhance CS-damaged counteract CS human cells. NCI-H727 non small cell carcinoma cells were transfected with plasmid vector expressing fused Enhanced Green Fluorescent Protein (EGFP). Cells fusion protein EGFP-FPG displayed accelerated adducts breaks induced by condensate. mutant frequencies low concentrations condensate Na(+)K(+)-ATPase locus (oua(r)) significantly reduced EGFP-FPG. Hence, stably protects from improving cells' capacity damaged DNA.