Sox9 Potentiates BMP2-Induced Chondrogenic Differentiation and Inhibits BMP2-Induced Osteogenic Differentiation

• DOI: 10.1371/journal.pone.0089025
• Publication Date: 2014-02-13T17:22:21Z
• Authors: Junyi Liao; Ning Hu; Nian Zhou; Liangbo Lin; Chen Zhao; Shixiong Yi; Tingxu Fan; Wei Bao; Xi Liang; Hong Chen; Wei Xu; Cheng Chen; Qiang Cheng; Yongming Zeng; Weike Si; Zhong Yang; Wei Huang
• Keywords: Endochondral ossification; Chondrogenesis; SOX9

Bone morphogenetic protein 2 (BMP2) is one of the key chondrogenic growth factors involved in cartilage regeneration. However, it also exhibits osteogenic abilities and triggers endochondral ossification. Effective chondrogenesis inhibition BMP2-induced osteogenesis ossification can be achieved by directing mesenchymal stem cells (MSCs) towards chondrocyte lineage with chodrogenic factors, such as Sox9. Here we investigated effects Sox9 on differentiation MSCs. We found exogenous overexpression enhanced MSCs vitro. Also, inhibited early late Subcutaneous cell implantation demonstrated potentiated formation Mouse limb cultures indicated that BMP2 acted synergistically to stimulate chondrocytes proliferation, hypertrophy This study strongly suggests potentiates formation, inhibits Thus, may a new strategy for tissue engineering.