Quantifying Insulin Sensitivity and Entero-Insular Responsiveness to Hyper- and Hypoglycemia in Ferrets

• DOI: 10.1371/journal.pone.0090519
• Publication Date: 2014-03-03T21:57:22Z
• Authors: Hongshu Sui; Yaling Yi; Jianrong Yao; Bo Liang; Xingshen Sun; Shanming Hu; Aliye Uc; Deborah J. Nelson; Katie Larson Ode; Louis H. Philipson; John F. Engelhardt; Andrew W. Norris
• Keywords: Clamp; Pathophysiology

Ferrets are an important emerging model of cystic fibrosis related diabetes. However, there is little documented experience in the use advanced techniques to quantify aspects diabetes pathophysiology ferret. Glycemic clamps gold standard technique assess both insulin sensitivity and secretion humans animal models We therefore sought develop for glycemic ferrets. To sensitivity, we performed euglycemic hyperinsulinemic 5–6 week old ferrets anesthetized conscious states. secretion, hyperglycemic evaluate responsiveness ferret islet entero-insular hormones low glucose, a portion were followed by hypoglycemic clamp. The demonstrated similar that previously observed rats. anesthetic isoflurane induced marked resistance, whereas lipid emulsion mild resistance. In ferrets, glucose appearance was largely suppressed at 4 mU/kg/min infusion, disposal progressively increased 20 insulin. Hyperglycemic clamp first phase secretion. Hypoglycemia rapid diminishment insulin, as well rise glucagon pancreatic polypeptide levels. incretins GLP-1 GIP affected minimally These will prove useful better defining with