Multiple Low-Dose Radiation Prevents Type 2 Diabetes-Induced Renal Damage through Attenuation of Dyslipidemia and Insulin Resistance and Subsequent Renal Inflammation and Oxidative Stress

• DOI: 10.1371/journal.pone.0092574
• Publication Date: 2014-03-20T21:08:49Z
• Authors: Minglong Shao; Xuemian Lu; Weitao Cong; Xiao Xing; Yi Tan; Yunqian Li; Xiaokun Li; Litai Jin; Xiaojie Wang; Juancong Dong; Shunzi Jin; Chi Zhang; Lu Cai
• Keywords: Dyslipidemia; Lipotoxicity

Background Dyslipidemia and lipotoxicity-induced insulin resistance, inflammation oxidative stress are the key pathogeneses of renal damage in type 2 diabetes. Increasing evidence shows that whole-body low dose radiation (LDR) plays a critical role attenuating stress. Objective The aims present study were to investigate whether LDR can prevent diabetes-induced underlying mechanisms. Methods Mice fed with high-fat diet (HFD, 40% calories from fat) for 12 weeks induce obesity followed by single intraperitoneal injection streptozotocin (STZ, 50 mg/kg) develop diabetic mouse model. mice exposed at different doses (25, 75 mGy) 4 or 8 along HFD treatment. At each time-point, kidney weight, function, blood glucose level resistance examined. pathological changes, lipid profiles, inflammation, fibrosis also measured. Results HFD/STZ-induced exhibited severe changes dysfunction. Exposure weeks, especially mGy, significantly improved sensitivity protein kinase B activation, meanwhile, attenuated kidney. LDR-induced anti-oxidative effect was associated up-regulation nuclear factor E2-related factor-2 (Nrf-2) expression function. However, above beneficial effects weakened once treatment extended weeks. Conclusion These results suggest exposure prevented injury characterized dysfunction changes. protective mechanisms complicated but may be mainly attributed attenuation dyslipidemia subsequent