Interleukin-19 Impairment in Active Crohn’s Disease Patients

• DOI: 10.1371/journal.pone.0093910
• Publication Date: 2014-04-09T20:56:54Z
• Authors: Elisabet Cantó; Esther Garcia Pla...; Carlos Zamora-Atenza; Juan Camilo Nieto; Jordi Gordillo; Ma Angels Ortiz; Isidoro Metón; Elena Serrano; Esteban Vegas; Orlando García-Bosch; Cándido Juárez; Sílvia Vidal
• Keywords: Adult; Male; 0301 basic medicine; Micro RNAs; Science; T cells; Lymphocyte Activation; Monocytes; 03 medical and health sciences; Th2 Cells; Malaltia de Crohn; Resposta immunitària; Crohn Disease; Citoquines; Humans; CTLA-4 Antigen; Flow cytometry; Immune response; Leucocytes; Cells, Cultured; Aged; Leucòcits; Gene Expression Profiling; Interleukins; Q; Toll-Like Receptors; R; Middle Aged; Recombinant Proteins; Interleukin-10; 3. Good health; Crohn's disease; MicroRNAs; Cèl·lules T; Gene Expression Regulation; Citometria de fluxe; Leukocytes, Mononuclear; Medicine; RNA; Cytokines; Female; Research Article

The exact function of interleukin-19 (IL-19) on immune response is poorly understood. In mice, IL-19 up-regulates TNFα and IL-6 expression its deficiency increases susceptibility to DSS-induced colitis. humans, favors a Th2 elevated in several diseases. We here investigate the effects cells from active Crohn’s disease (CD) patient. Twenty-three CD patients 20 healthy controls (HC) were included. mRNA protein levels analyzed monocytes. determined vitro T cell phenotype production cytokines by cells. observed that unstimulated TLR-activated monocytes expressed significantly lower than HC (logFC = −1.97 unstimulated; −1.88 with Pam3CSK4; −1.91 FSL-1; p<0.001). These results confirmed at level. Exogenous had an anti-inflammatory effect but not patients. decreased PBMC (850.7±75.29 pg/ml vs 2626.0±350 pg/ml; p<0.01) increased CTLA4 (22.04±1.55% 13.98±2.05%; p<0.05) IL-4 (32.5±8.9 13.5±2.9 HC. IL-10 regulated both three miRNAs can modulate expression, up-regulated suggested role this study. Defects lack cytokine could contribute inflammatory mechanisms