Evaluation of Cancer Stem Cell Markers CD133, CD44, CD24: Association with AKT Isoforms and Radiation Resistance in Colon Cancer Cells

• DOI: 10.1371/journal.pone.0094621
• Publication Date: 2014-04-23T22:26:38Z
• Authors: Sara Häggblad Sah...; Diana Spiegelberg; Bengt Glimelius; Bo Stenerlöw; Marika Nestor
• Keywords: AKT2; CD24; Stem cell marker

The cell surface proteins CD133, CD24 and CD44 are putative markers for cancer stem populations in colon cancer, associated with aggressive types poor prognosis. It is important to understand how these may predict treatment outcomes, determined by factors such as radioresistance. scope of this study was assess the connection between EGFR, CD24, (including isoforms) expression levels radiation sensitivity, furthermore analyze influence AKT isoforms on patterns markers, better underlying molecular mechanisms cell. Three cell-lines were used, HT-29, DLD-1, HCT116, together DLD-1 isogenic knock-out cell-lines. All three (HT-29, HCT116 DLD-1) expressed varying amounts top ten percent CD133 expressing cells (CD133high/CD44high) more resistant gamma than lowest (CD133low/CD44low). lower fraction low CD133/CD44. Depletion AKT1 or AKT2 using knock out showed first time that but not AKT2, whereas influenced presence either AKT2. There several genes adhesion pathway which had significantly higher KO cell-line compared cell-line; however epithelial mesenchymal transition (CDH1, VIM, TWIST1, SNAI1, SNAI2, ZEB1, ZEB2, FN1, FOXC2 CDH2) did differ. Our results demonstrate CD133high/CD44high increased resistance, different have effects an consideration when targeting a clinical setting.