Growth Inhibitory, Bactericidal, and Morphostructural Effects of Dehydrocostus Lactone from Magnolia sieboldii Leaves on Antibiotic-Susceptible and -Resistant Strains of Helicobacter pylori

• DOI: 10.1371/journal.pone.0095530
• Publication Date: 2014-04-19T00:09:48Z
• Authors: Hyun-Kyung Lee; Ha Eun Song; Haeng-Byung Lee; Cheol-Soo Kim; Mamoru Koketsu; Luong Thi My Ngan; Young-Joon Ahn
• Keywords: Sesquiterpene lactone

Helicobacter pylori is associated with various diseases of the upper gastrointestinal tract, such as gastric inflammation and duodenal ulcers. The aim study was to assess anti-H. effects sesquiterpene lactone dehydrocostus (DCL) from Magnolia sieboldii leaves, compared commercial pure DCL, two previously known lactones (costunolide parthenolide), (–)-epigallocatechin gallate, four antibiotics. antibacterial activity natural DCL toward antibiotic-susceptible H. ATCC 700392 700824 strains (MIC, 4.9 4.4 mg/L) similar that more effective than costunolide, parthenolide, EGCG. slightly lower metronidazole 1.10 1.07 mg/L). virtually identical susceptible resistant strains, even though resistance amoxicillin 11.1 mg/L for PED 503G strain), clarithromycin (49.8 3582GA (21.6 43504 strain; 71.1 221 or tetracycline (14.2 B strain) observed. This finding indicates antibiotics do not share a common mode action. bactericidal affected by pH values examined (4.0–7.0). caused considerable conversion coccoid form (94 versus 49% at 8 4 48 h). Western blot analysis revealed urease subunits (UreA UreB) were 10 mM whereas UreA monomer band completely disappeared 0.1 gallate. Global efforts reduce level justify further studies on M. leaf-derived materials containing potential products lead molecule prevention eradication drug-resistant pylori.