Development of an Allele-Specific PCR Assay for Simultaneous Sero-Typing of Avian Pathogenic Escherichia coli Predominant O1, O2, O18 and O78 Strains

• DOI: 10.1371/journal.pone.0096904
• Publication Date: 2014-05-07T20:40:38Z
• Authors: Shaohui Wang; Qingmei Meng; Jianjun Dai; Xiangan Han; Yue Han; Chan Ding; Haiwen Liu; Shengqing Yu
• Keywords: Pathogenic Escherichia coli; Agglutination (biology)

Systemic infections by avian pathogenic Escherichia coli (APEC) are economically devastating to poultry industries worldwide. E. strains belonging serotypes O1, O2, O18 and O78 preferentially associated with colibacillosis. The rfb gene cluster controlling O antigen synthesis is usually various among different serotypes. In present study, the clusters of were characterized compared. Based on serotype-specific genes in cluster, an allele-specific polymerase chain reaction (PCR) assay was developed. This PCR highly specific reliable for sero-typing APEC strains. sensitivity determined as 10 pg DNA or colony forming units (CFUs) bacteria O2 strains, 500 1,000 CFUs O1 Using this system, isolates infected tissue samples categorized successfully. Furthermore, it able differentiate multi-agglutination traditional serum agglutination assay. Therefore, more simple, rapid accurate diagnosis, epidemiologic study vaccine development.