Hijacking of Host Cellular Functions by an Intracellular Parasite, the Microsporidian Anncaliia algerae

• DOI: 10.1371/journal.pone.0100791
• Publication Date: 2014-06-26T20:59:29Z
• Authors: Johan Panek; Hicham El Alaoui; Anne Mone; Serge Urbach; Edith Demettre; Catherine Texier; Christine Brun; Andreas Zanzoni; Eric Peyretaillade; Nicolas Parisot; Emmanuelle Lerat; Pierre Peyret; Frederic Delbac; David G. Biron
• Keywords: Intracellular parasite; Obligate

Intracellular pathogens including bacteria, viruses and protozoa hijack host cell functions to access nutrients bypass cellular defenses immune responses. These strategies have been acquired through selective pressure allowed reach an appropriate niche for their survival growth. To get new insights on how parasites functions, we developed a SILAC (Stable Isotope Labeling by Amino Acids in Cell culture) quantitative proteomics workflow. Our study focused deciphering the cross-talk host-parasite association, involving human foreskin fibroblasts (HFF) microsporidia Anncaliia algerae, fungus related parasite with obligate intracellular lifestyle strong dependency. The was analyzed at five post-infection times 1, 6, 12 24 hours (hpi) 8 days (dpi). A significant up-regulation of four interferon-induced proteins tetratricopeptide repeats IFIT1, IFIT2, IFIT3 MX1 observed dpi suggesting type 1 interferon (IFN) response. Quantitative alteration involved biological such as signaling (STAT1, Ras) reduction translation activity (EIF3) confirmed IFN Interestingly, approach also detection 148 A. algerae during kinetics infection. Among these many are proliferation, over-representation putative secreted effectors observed. Finally our survey suggests that could use transposable element lure strategy escape innate system.