MicroRNA Expression Differences in Human Hematopoietic Cell Lineages Enable Regulated Transgene Expression

• DOI: 10.1371/journal.pone.0102259
• Publication Date: 2014-07-16T17:40:15Z
• Authors: Raul Teruel-Montoya; Xianguo Kong; Shaji Abraham; Lin Ma; Satya P. Kunapuli; Michael Holinstat; Chad A. Shaw; Steven E. McKenzie; Leonard C. Edelstein; Paul F. Bray
• Keywords: Jurkat cells; Blood cell

Blood microRNA (miRNA) levels have been associated with and shown to participate in disease pathophysiology. However, the hematopoietic cell of origin blood miRNAs individual miRNA profiles are poorly understood. We report content highly purified normal cells from same individuals. Although T-cells, B-cells granulocytes had highest per cell, erythrocytes contributed more cellular blood, followed by platelets. profiling revealed different patterns expression specific for each lineage. miR-30c-5p was determined be an appropriate reference normalizer cross-cell qRT-PCR comparisons. 5 lines differential miR-125a-5p. demonstrated endogenous miR-125a-5p regulate reporter gene Meg-01 Jurkat (1) constructs containing binding sites or (2) over-expressing inhibiting This quantitative analysis peripheral identifies circulating miRNAs, supports use distinguishing lineages suggests that endogenously expressed can exploited transgene a cell-specific manner.