Flow Characterization of a Spinner Flask for Induced Pluripotent Stem Cell Culture Application

• DOI: 10.1371/journal.pone.0106493
• Publication Date: 2014-10-03T17:46:11Z
• Authors: Mohd-Zulhilmi Ismadi; Priyanka Gupta; Andreas Fouras; Paul Verma; Sameer Jadhav; Jayesh Bellare; Kerry Hourigan
• Keywords: Microcarrier

We present detailed quantitative measurement analyses for flow in a spinner flask with spinning rates between 20 to 45 RPM, utilizing the optical velocimetry technique of Particle Image Velocimetry (PIV). A partial section impeller was immersed working fluid reduce shear forces induced on cells cultured microcarriers. Higher rotational speeds improved mixing effect medium at expense higher environment. It found that mouse pluripotent stem (iPS) achieved optimum number over 7 days 25 RPM suspension culture. This condition translates 0.0984 Pa maximum stress caused by interaction bottom surface. However, inverse cell growth obtained 28 culture condition. Such narrow margin demonstrated iPS microcarriers are very sensitive mechanical forces. study provides insight biomechanical parameters, specifically distribution, commercially available wide range Reynolds number.