Erythrocyte Phospholipid and Polyunsaturated Fatty Acid Composition in Diabetic Retinopathy

• DOI: 10.1371/journal.pone.0106912
• Publication Date: 2014-09-05T02:17:26Z
• Authors: Philippe Koehrer; Sarah Saab; Olivier Berdeaux; Rodica Isaïco; Stéphane Grégoire; Stéphanie Cabaret; Alain M. Bron; Catherine P. Creu...; Lionel Bretillon; Niyazi Acar
• Keywords: EXPRESSION; Adult; Male; Organes des sens; Science; Sensory Organs; Plasmalogens; 610; Médecine humaine et pathologie; Severity of Illness Index; MELLITUS; 03 medical and health sciences; Humans; OXIDATIVE STRESS; [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs; RETINA; Phospholipids; Aged; COMPLICATIONS; 0303 health sciences; Diabetic Retinopathy; INSULIN SENSITIVITY; Phosphatidylethanolamines; Q; Erythrocyte Membrane; R; DOCOSAHEXAENOIC ACID; Middle Aged; ENDOTHELIAL-CELLS; 3. Good health; DOCOSAHEXAENOIC ACID;INSULIN SENSITIVITY;ENDOTHELIAL-CELLS;OXIDATIVE STRESS;RETINA;LIPIDS;DEFORMABILITY;COMPLICATIONS;EXPRESSION;MELLITUS; Diabetes Mellitus, Type 1; Diabetes Mellitus, Type 2; Case-Control Studies; Fatty Acids, Unsaturated; Phosphatidylcholines; Medicine; Human health and pathology; Female; [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology; LIPIDS; DEFORMABILITY; Research Article

Background Long chain polyunsaturated fatty acids (LCPUFAs) including docosahexaenoic acid and arachidonic are suspected to play a key role in the pathogenesis of diabetes. LCPUFAs known be preferentially concentrated specific phospholipids termed as plasmalogens. This study was aimed highlight potential changes metabolism phospholipids, particularly plasmalogens, at various stages diabetic retinopathy humans. Methodology Principal Findings We performed lipidomic analyses on red blood cell membranes from controls mainly type 2 diabetes mellitus patients with or without retinopathy. The composition erythrocytes determined by gas chromatography phospholipid structure liquid equipped an electrospray ionisation source coupled tandem mass spectrometer (LC-ESI-MS/MS). A significant decrease levels observed. origin this loss phosphatidyl-ethanolamine esterified these LCPUFAs. In retinopathy, change balanced increase several phosphatidyl-choline species. No influence nor observed concentrations plasmalogen-type phospholipids. Conclusions Significance Diabetes were associated reduction erythrocyte phosphatidyl-ethanolamines. amounts species might compensatory mechanism for LC-PUFA-rich