Recombinant p35 from Bacteria Can Form Interleukin (IL-)12, but Not IL-35

• DOI: 10.1371/journal.pone.0107990
• Publication Date: 2014-09-26T18:35:48Z
• Authors: Samadhi Aparicio-...; Jens M. Moll; Juliane Lokau; Melanie Grusdat; Jutta Schröder; Svenja Plöhn; Stefan Rose-John; Joachim Grötzinger; Philipp A. Lang; Jürgen Scheller; Christoph Garbers
• Keywords: Glycoprotein 130; HEK 293 cells

The Interleukin (IL)-12 family contains several heterodimeric composite cytokines which share subunits among each other. IL-12 consists of the p40 (shared with IL-23) and p35. p35 is shared cytokine IL-35 comprises p35/EBI3 heterodimer (EBI3 IL-27). signals via homo- or heterodimers IL-12Rβ2, gp130 WSX-1, are IL-27 receptor complexes, respectively. was efficiently secreted in complex as but not EBI3 transfected cell lines tested complicates analysis signal transduction. form an inter-chain disulfide bridge. Mutation responsible cysteine residue (p40C197A) reduced formation activity only slightly. Importantly, p40C197A mutation prevented antagonistic homodimers enabled vitro reconstitution biologically active produced bacteria (p35bac). Reconstitution p35bac did, however, fail to induce transduction Ba/F3 cells expressing IL-12Rβ2 gp130. In summary, we describe IL-12, produce recombinant by this novel approach.