Human Cytomegalovirus Tegument Protein pp65 Is Detected in All Intra- and Extra-Axial Brain Tumours Independent of the Tumour Type or Grade

• DOI: 10.1371/journal.pone.0108861
• Publication Date: 2014-09-30T19:32:53Z
• Authors: Sylwia Libard; Svetlana N. Popova; Rose-Marie Amini; Vesa Kärjä; Timo Pietiläinen; Kirsi M. Hämäläinen; Christer Sundström; Göran Hesselager; Michael Bergqvist; Simon Ekman; Maria Zetterling; Anja Smits; Pelle Nilsson; Susan Pfeifer; Teresita Diaz de ...; Gunilla Enblad; Fredrik Ponten; Irina Alafuzoff
• Keywords: Tissue microarray; Human brain

Human cytomegalovirus (HCMV) has been indicated being a significant oncomodulator. Recent reports have suggested that an antiviral treatment alters the outcome of glioblastoma. We analysed performance commercial HCMV-antibodies applying immunohistochemical (IHC) methods on brain sample obtained from subject with verified HCMV infection, samples 14 control subjects, and tissue microarray block containing cores various tumours. Based these trials, we selected best performing antibody cohort 417 extra- intra-axial tumours such as gliomas, medulloblastomas, primary diffuse large B-cell lymphomas, meningiomas. protein pp65 immunoreactivity was observed in all types analysed, IHC expression did not depend patient's age, gender, tumour type, or grade. The labelling pattern differed active infection. expressed up to 90% investigated. Our results are accordance previous regarding glioblastomas medulloblastomas. In addition, seen low-grade indicate is common intra- extra-axial Thus, assessment origins pathologically altered conditions inflammation, even degeneration should certainly be facilitated.