Mimotope-Based Vaccines of Leishmania infantum Antigens and Their Protective Efficacy against Visceral Leishmaniasis

• DOI: 10.1371/journal.pone.0110014
• Publication Date: 2014-10-21T16:03:42Z
• Authors: Lourena Emanuele ...; Luiz Ricardo Goulart; Nathália Cristina...; Mayara Ingrid Sou...; Mariana Costa Duarte; Vivian Tamietti M...; Paula Sousa Lage; Daniel Menezes-Souza; Tatiana Gomes Rib...; Maria Norma Melo; Ana Paula Fernandes; Manuel Soto; Carlos Alberto Pe...; Miguel Angel Cháv...; Eduardo Antonio F...
• Keywords: Mimotope

The development of cost-effective prophylactic strategies to prevent leishmaniasis has become a high-priority. present study used the phage display technology identify new immunogens, which were evaluated as vaccines in murine model visceral (VL). Epitope-based represented by phage-fused peptides that mimic Leishmania infantum antigens, selected according their affinity antibodies from asymptomatic and symptomatic VL dogs' sera.Twenty clones after three selection cycles, means vitro assays immune stimulation spleen cells derived naive chronically infected with L. BALB/c mice. Clones able induce specific Th1 response, high levels IFN-γ low IL-4 selected, based on selectivity specificity, two clones, namely B10 C01, further employed vaccination protocols. mice vaccinated plus saponin showed both production IFN-γ, IL-12, GM-CSF individual or extracts. Additionally, these animals, when compared control groups (saline, saponin, wild-type non-relevant clone saponin), significant reductions parasite burden liver, spleen, bone marrow, paws' draining lymph nodes. Protection was associated an IL-12-dependent mainly CD8+ T cells, against proteins. These animals also presented decreased parasite-mediated IL-10 responses, increased parasite-specific IgG2a antibodies.This describes directly immunogens Th1-type significantly reduced burden. This is first phage-displayed successful vaccine formulations VL.