Proangiogenic Compositions of Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells

• DOI: 10.1371/journal.pone.0115316
• Publication Date: 2014-12-16T19:28:13Z
• Authors: Jianying Chen; Zhenjun Liu; Mian Ming Hong; Hongzhe Zhang; Can Chen; Mengyuan Xiao; Junxian Wang; Feng Yao; Mingchuan Ba; Jinghu Liu; Zi-Kuan Guo; Jixin Zhong
• Keywords: Angiogenin

Introduction & Objective Microvesicles (MVs) derived from mesenchymal stem cells (MSCs) have been shown to promote angiogenesis. This study was aimed shed a light on the mechanisms by analyzing angiogenesis-promoting compositions of MSC-MVs. Also we try figure out impact hypoxia Methods MVs were isolated culture supernatants MSCs under hypoxia/normoxia and serum-deprivation condition. The morphological features revealed an electron microscope origin identified bead-bound assay. An antibody array used analyze expression angiogenic cytokines parent as well. major candidate factors screened results validated immune blotting. Results MSC-MVs around 80 nm in diameter. They expressed CD29, CD44, CD73, but not CD31 CD45. Antibody showed that both many biomolecules, including interleukin-6 (IL-6), basic fibroblast growth (bFGF), recptor urokinase-type plasminogen activator (UPAR). contained angiogenin, vascular endothelial factor (VEGF), monocyte chemotactic protein-1 (MCP-1) receptor-2 for at higher levels than MSCs. Under hypoxic condition most greater quantity normoxic while there no significant difference between conditions except UPAR, Angiogenin, VEGF, IGF, Tie-2/TEK, IL-6 which those Conclusion Upon condition, could secrete contain variety contributing their function. And among them, MCP-1, VEGF R2 might be importance other cytokines. responsible hypoxia-augmented proangiogenic effects MVs.